中文摘要：

目的应用RNA干扰技术降低促凋亡基因bax的表达，以阻抑铝诱导的神经胶质瘤细胞凋亡。方法设计了3对阻抑bax基因表达的siRNA序列，对神经胶质瘤细胞进行了转染，根据不同siRNA序列在不同转染剂量、不同转染时间的细胞活力，找到bax siRNA转染的最佳转染剂量和最适转染时间。依据RNA干扰前后bax基因的表达变化筛检出最有效的siRNA序列。用荧光染色法、荧光定量PCR法和免疫组织化学染色法分别检测其转染效率、对基因表达的干扰效率及对蛋白表达的阻抑效应。结果最有效的针对bax基因的siRNA序列为siRNAl，该序列的转染效率〉90％，对bax基因表达的干扰效率为62.3％，最佳转染后检测点为转染后72h，最适转染剂量为20nmol／L。结论阻抑bax基因表达的siRNA序列能有效干扰bax基因和蛋白的表达，降低凋亡率。

英文摘要：

Objectlve To decrease bax expression by RNA interference, and inhibite the apoptosis in glioma cells induced by aluminum. Methods We had designed three siRNA sequences, and transfected them into glioma cells, The optimal sequence was selected based on the results of cell viability at various transfection concentrations and different transfection times. Fluorescentstaining was used to detect the transfection efficiency, interference efficiency was determined by QRT-PCR method, and protein expression was measured with immunohistochemistry. Results The optimal siRNA sequence was selected based on the cell viability. The transfection efficiency was above 90% , the interference efficiency of bax gene was 62.3 % , and the optimal transfection time was 72h after transfection, the optimal transfection concentration was 20nmol/L, besides, there was a significant decrease in Bax protein content after transfection. Conclusion The optimal bax siRNA sequence can effectively inhibit the expression of bax gene and Bax protein, which may decrease apoptosis in aluminum-induced toxicity.