中文摘要：

Nestin，中间的细丝蛋白质，在发展的神经干细胞被表示中央神经系统。这织物特定的表情被神经的茎驾驶在在基因的巢的第二 intron 的房间特定的 enhancer。在这研究，我们证明在基因的老鼠巢在 pluripotent 被表示胚胎的癌(EC ) P19 和 F9 房间，不是在区分的房间类型。这个房间类型特定的表示被 enhancer 在第二 intron 授与。在 enhancer 的保存 POU 因素绑定地点的变化在 EC 房间废除了记者基因表示。Oct4，一个班 V POU 因素，被发现是有巢在的 coexpressed 在 EC 房间。Electrophoretic 活动性移动试金和超级移动试金证明唯一的 protein-DNA 建筑群与 EC 房间的原子摘录明确地被形成，并且 Oct4 蛋白质被包括。一起，这些结果在 pluripotent EC 房间在基因建议在保存 POU 因素绑定地点和巢的表示之间的功能的关联。

英文摘要：

Nestin, an intermediate filament protein, is expressed in the neural stem cells of the developing central nervous system. This tissue-specific expression is driven by the neural stem cell-specific enhancer in the second intron of the nestin gene. In this study, we showed that the mouse nestin gene was expressed in pluripotent embryonic carcinoma （EC） P19 and F9 cells, not in the differentiated cell types. This cell typespecific expression was conferred by the enhancer in the second intron. Mutation of the conserved POU factor-binding site in the enhancer abolished the reporter gene expression in EC cells. Oct4, a Class V POU factor, was found to be coexpressed with nestin in EC cells. Electrophoretic mobility-shift assays and supershift assays showed that a unique protein-DNA complex was formed specifically with nuclear extracts of EC cells, and Oct4 protein was included. Together, these results suggest the functional relevance between the conserved POU factor-binding site and the expression of the nestin gene in pluripotent EC cells.