中文摘要：

研究了产紫青霉Penicillium purpurogenumLi-3全细胞β-D-葡萄糖醛酸苷酶在水/有机溶剂体系中催化水解甘草酸,合成单葡糖醛酸基甘草次酸（GAMG）的反应。确定了最适反应体系为水/叔丁醇（体积比为3∶7）,最适反应条件为：底物甘草酸用量1.5g·L^-1、反应温度45℃、pH值5.0、全细胞酶量7g·L^-1、摇床转速120r·min^-1,在此条件下反应48h,产物GAMG产率可达79.12%,全细胞酶具有较好的稳定性和可重复使用性,显示了良好的应用前景。

英文摘要：

Biosynthesis of glycyrrhetinic acid monoglucuron whole cell of Penicillium purpurogenum Li-3 in an aqueous e （GAMG） by β-D-glucuronidase from the organic system was studied. Results showed that tert-butyl alcohol is the best organic solvent and the optimum volume ratio of aqueous to organic phase is 3 to 7. The rest optimum conditions for this biocatalysis are 1.5 g ·L^-1 of substrate concentration, 45℃ of temperature, pH 5.0, 7 g ·L^-1 of enzyme concentration and 120 r · min^-1 of shaking rate. Under these optimized conditions, 79.12% yield of GAMG was achieved after 48 h reaction. The whole-cell enzyme has a good reusability and a high stability, which demonstrates the prospect for real application.