中文摘要：

目的：建立同时测定通塞脉微丸中绿原酸、阿魏酸、甘草苷、肉桂酸4种成分的超高效液相色谱（UPLC）分析方法。方法：采用Waters AcquityUPLC系统,AcquityUPLC BEH C18（50 mm×2.1 mm,1.7μm）色谱柱,以乙腈-0.1%甲酸水为流动相,梯度洗脱,双波长检测（λ1=276 nm、λ2=327 nm）,柱温35℃。结果：绿原酸、阿魏酸、甘草苷、肉桂酸在16 min内即达到基线分离,在测定的浓度范围内线性关系良好（r≥0.9997）,各成分的平均回收率在95.1%～98.2%,RSD〈4.0%。结论：UPLC分离效果及重复性好,且快速、简便,可作为通塞脉微丸的质量控制方法。

英文摘要：

Objective：To establish a ultra performance liquid chromatography（UPLC） method for simultaneous determination of 4 components—chlorogenic acid,ferulic acid,liquiritin and cinnamic acid in Tongsaimai pellets.Methods：The analyses were performed on a Waters AcquityUPLC system equipped with a binary solventmanager,a samplermanager,a column compartment and a PDA detector,connected to Waters Empower 2 software.An AcquityUPLC BEH C18 column（50 mm×2.1 mm,1.7 μm）was used for all analyses.The investigated compounds were separated with gradient mobile phase consisting of acetomitrile-0.1% formic acid.Chlorogenic acid,ferulic acid,liquiritin and cinnamic acid were determined by dual wavelength of 276 nm（λ1） and 327 nm（λ2）,and column temperature 35 ℃.Results：Four components could be well separated at the baseline in 16 min.The average recovery was 95.1% to 98.2% with RSD4.0%.Conclusion：The developed UPLC method is simple,sensitive and accurate with good repeatability,which is available for quality control of Tongsaimai pellets.