通过盆栽试验研究了Glomus geosporum,G.mosseae,G.versiforme,G.etunicatum以及G.diaphanum等5种丛枝菌根真菌(AMF)对核素铯污染胁迫下宿根高粱根系抗坏血酸-谷胱甘肽(As A-GSH)循环的影响。结果表明,接种G.mosseae,G.versiforme和G.etunicatum处理显著增加了核素铯污染胁迫下宿根高粱根系还原性抗坏血酸(As A)和还原性谷胱甘肽(GSH)含量,同时,3种处理还显著提高了As A/DHA和GSH/GSSG比值;另外,5种菌根处理均提高了As A-GSH循环酶活性,其中抗坏血酸过氧化物酶(APX)、双脱氢抗坏血酸还原酶(DHAR)和谷胱甘肽还原酶(GR)以G.mosseae处理效果最好,其活性分别相当于对照的4.74,3.63和2.34倍,而单脱氢抗坏血酸还原酶(MDHAR)活性以接种G.versiforme处理增加最大,与对照相比增加了2.39 U/g FW,说明AMF能促进铯污染下宿根高粱根系As A-GSH循环快速有效地运转,增强植物耐核素铯的能力。
A pot experiment was conducted to study the effects of Glomus geosporum, G. mosseae, G. versiforme, G. etunicatum and G. diaphanum on ascorbate-glutathione cycle metabolism in roots of Sorghum haipense at soil contaminated under Cs stress. The study showed that AsA and GSH content was significantly increased by inoculation of G.mosseae, G. versiforme and G.etunicatum; meanwhile, the ratios of AsA/DHA and GSH/GSSG in roots of Sorghum haipense in soil contaminated with Cs were increased as well. Additionally, treatment by five mycorrhizas greatly upgraded the enzyme activities of ascorbate-glutathione cycle, among others G. mossea was the best, and the activities of antioxidant enzymes (APX, DHAR and GR)were respectively 4.74, 3.63 and 2.34 times higher than those of control ones, while for MDHAR activity the best was that treated by G.versiforme with an increase of 2.39 U/gFW than the control one. In conclusion, AMF- inoculation in the root of Sorghum haipense could enhance efficient metabolism of ascorbate-glutathione cycle, being conducive to a stronger tolerance of Sorghum haipense to Cs stress.