通过盆栽试验研究了接种透光球囊霉( Glomus diaphanum)、摩西球囊霉( Glomus mosseae)、地表球囊霉(Glomus versiforme)及幼套球囊霉(Glomus etunicatum)等4种丛枝菌根真菌(AMF)对铯污染下宿根高粱生长及根际土壤酶活性的影响.结果表明,与未接种对照相比,接种AMF处理均显著增加了宿根高粱的株高和根长,同时增强了过氧化氢酶、蔗糖酶活性及根系活力(P<0.05),且以接种G.etunicatum增强效果最好,上述酶活性和根系活力分别相当于对照的1.11、2.25和4.04倍,而接种Glomus diaphanum最显著增强了酸性磷酸酶活性,使其提高了24.53 mg·g^-1·d^-1·FW(P<0.05),宿根高粱对铯的耐性指数均高于对照,说明AMF在一定程度上能缓解铯污染胁迫对根际土壤酶的抑制作用,进而减轻核素铯对植物的毒害;接种AMF处理显著抑制了脲酶活性,表明脲酶可作为反应土壤核素铯污染的一个有效指标.同时也表明AMF有效地增强了宿根高粱对核素铯的耐性能力.
A pot experiment was conducted to investigate the effects of Glomus diaphanum, G.mosseae, G. versiforme and G. etunicatum on growth of Sorghum haipense and rhizosphere soil enzymatic activities under Cs stress. The results showed that, AMF-inoculation significantly increased the stem diameter and root length of Sorghum haipense, enchanced catalase and invertase enzyme activities as well as root activities. Compared to control, better efficiency was shown in G.etunicatum, and the activities were respectively 1.11,2.25 and 4.04 times higher than those of non-inoculated treatments. Sorghum haipense inoculated with Glomus diaphanum increased acid phosphatase activity by 24.53 mg·g^-1·d^-1·FW( P〈0.05) under cesium stress. The tolerance index of Sorghum haipense inoculated with AMF was higher than control, which demonstrated that AMF-inoculation significantly counteracted the inhibition of Cs pollution to rhizosphere soil enzymes activities, and then relieved Cs toxicity to plants. AMF-inoculation significantly inhibited urease activities in rhizosphere soil(P〈0.05), so urease could be used as an effective indicator for Cs contamination in soil. Meanwhile, it demonstrated that AMF-inoculation efficiently increased the tolerance capacity of Sorghum haipense to Cs stress.