中文摘要：

种子细胞是影响组织工程发展的关键因素之一，如何获得和运用理想的种子细胞是当今组织工程与再生医学研究的焦点和热点。干细胞具有很强的自我更新能力和多向分化能力，作为组织工程的种子细胞有着巨大的应用潜力。本文针对胚胎干细胞、间充质干细胞和诱导多能干细胞等组织工程潜在的种子细胞，就其最新研究进展及应用做一综述，并展望了它们作为种子细胞的前景和目前面临的困难。

英文摘要：

Seeding cells is one of key factors in the development of tissue engineering. How to gain and use the seeding cells became a hot issue of the research on the tissue engineering and regenerative medicine. Stem cells have great abilities of self-renewing and pluripotent differentiation, which is supposed to be the ideal candidates in engineering of tissue or/and organ. In this paper, we briefly summarized the recent research progress of some widely used stem cells such as embryonic stem cell, mesenchymal stem cells and induced pluripotent stem cells, and reviews their prospects and difficulty to be seeded cells in tissue engineering research.