中文摘要：

以鬼臼毒素（Podophyllotoxin,PPT）为模板,甲基丙烯酸（Methacrylic acid,MAA）为功能单体,乙二醇二甲基丙烯酸酯（Ethyleneglycol dimethacrylate,EGDMA）为交联剂,固定PPT,MAA和EGDMA的摩尔比为1∶2∶100,采用原位聚合法,以甲醇-乙酸（1∶1,V/V）为洗脱剂,制得鬼臼毒素分子印迹电化学传感器（PPT molecularly imprinted electrochemical sensor,PPT-MIECS）,并用循环伏安及交流阻抗法表征了其性能。在优化条件下,此传感器检测PPT的线性范围为1.0-120$mol/L,检出限为0.47$mol/L,将其用于桃儿七和人血清样品中PPT的检测,相对标准偏差小于4.0%,加标回收率在95.0%%103.3%之间。

英文摘要：

A novel electrochemical sensor for the determination of podophyllotoxin（ PPT） was prepared by modifying a PPT-imprinted membrane,which was synthesized by in situ polymerization with PPT as template, methacrylic acid（ MAA） as functional monomer and ethylene glycol dimethacrylate（ EGDMA） as cross linking agent at the molar ratio of PPT / MAA / EGDMA as 1∶ 2∶ 100,on glassy carbon electrode（ GCE） followed by eluting PPT from the modified GCE with methanol / acetic acid（1∶ 1,V / V）. The properties of the electrochemical sensor modified with PPT-imprinted membrane were characterized by cyclic voltammetry（CV）,electrochemical impedance spectroscopy（EIS）. Under the optimum conditions,it was found that the response of peak currents was linear to the concentration of PPT in range of 1. 0- 120 μmol / L with the detection limit of 0. 47 μmol / L. The sensor not only showed high selectivity,but also exhibited good stability and reproducibility. The sensor was satisfactorily applied to the determination of PPT in podophyllum hexandrum and human serum samples with the relative standard deviation（ RSD） below 3. 9% and recovery ranging from 95. 0% to 103. 3%.