中文摘要：

目的研究忍冬Lonicera japonica根中的化学成分及其抗炎活性。方法采用反复碱性硅胶柱色谱、Sephadex LH-20柱色谱、薄层色谱、制备液相色谱等方法进行分离纯化,并通过波谱学分析鉴定其化学结构;采用斑马鱼抗炎模型,测定分离所得部分单体成分的抗炎活性。结果从忍冬根95%乙醇提取物中分离得到14个化合物,并鉴定其结构为3,13-dihydroxystemodan-2-one（1）、大黄酚（2）、palmarumycin CP2（3）、β-谷甾醇（4）、豆甾醇（5）、豆甾-4,6,8（14）,22-四烯-3-酮（6）、erythrinassinate D（7）、羊毛甾醇（8）、齐墩果酸-3-乙酸酯（9）、原儿茶醛（10）、胡萝卜苷（11）、（E）-3-（3,4-二羟基苯亚甲基）-5-（3,4-二羟基苯）-2（3H）-呋喃酮（12）、lomacarinoside B（13）、（2E,6S）-8-（α-L-arabinopyranosyl-（1″→6′）-β-D-glucopyranosyloxy）-2,6-dimethyloct-2-eno-1,2″-lactone（14）。结论化合物1为新化合物,化合物2、3、6、7～9、13均为首次从该植物中分离得到;化合物9、10在100μg/m L质量浓度下表现出明显的抗炎活性。

英文摘要：

objective To research the chemical composition from the roots of Lonicera japonica and their anti-inflammatory activities. Methods The compounds were isolated and purified by chromatography on silica gel column, Sephadex LH-20 column,preparative thin-layer, and semi-preparative HPLC, etc. Their structures were identified on the basis of spectroscopic data, and parts of the isolated compounds were tested for their anti-inflammatory activities against zebrafish. Results Fourteen compounds were isolated and elucidated as 3,13-dihydroxystemodan-2-one（1）, chrysophanol（2）, palmarumycin CP2（3）, β-sitosterol（4）, stigmasterol（5）, stigmast-4,6,8（14）, 22-tetraen-3-one（6）, erythrinassinate D（7）, lanosterin（8）, 3-O-acetyloleanolic acid（9）, protocatechuin aldehyde（10）, daucosterol（11）,（E）-3-（3,4-dihydroxybenzylidene）-5-（3,4-dihydroxyphenyl）-2（3H）-furanone（12）, lomacarinoside B（13）, and（2E,6S）-8-（α-L-arabinopyranosyl-（1″→6′）-β-D-glucopyranosyloxy）-2,6-dimethyloct-2-eno-1,2″-lactone（14）. Conclusion Compound 1 is a new compound, and compounds 2, 3, 6, 7—9, and 13 are isolated from L. japonica for the first time. Compounds 9and 10 show the significant anti-inflammatory activities at 100 μg/m L.