中文摘要：

本文运用SPME-GC/MS联合HPLC-DAD法分析表征金银花新品种花和叶中的主要化学成分,为开发利用金银花新品种提供数据支持。采用SPME-GC/MS对该品种花及叶片中的挥发油成分进行检测;并采用HSDE-HPLC-DAD对该品种花及叶片中有机酸、黄酮、环烯醚萜苷类成分进行分析测定。最终从该品种金银花中检测出54种挥发油成分,定量测定出四种有机酸（绿原酸、隐绿原酸、异绿原酸A、异绿原酸C）、两种黄酮（芦丁、木犀草苷）、两种环烯醚萜苷（马钱酸、当药苷）共八个化合物的含量。该方法以金银花中四类主要成分为指标,全面系统的对该金银花新品种花及叶进行分析测定和质量评价,为新品种金银花的推广及应用提供科学依据。

英文摘要：

In this study,a comprehensive method was established to analyze and characterize the chemical ingredients in a new variety of Lonicera japonica by SPME-GC / MS coupled with HPLC-DAD. The volatile oils of the investigated sample were determined by GC / MS. Other organic acids,flavonoids and iridoids were analyzed by HSDE-HPLC-DAD. As a result,54 volatile oils were detected. In addition,the contents of four organic acids（ chlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A and isochlorogenic acid C）,two flavonoids（ rutin and galuteolin） and two iridoids（ loganic acid and chiratin） were determined. This study proposed a method for the comprehensively and systematically evaluation of germplasm by determining the 4 types of main ingredients in L. japonica. It will provide scientific basis for the popularization and further utility of L. japonica.