中文摘要：

2012年12月，安徽某些蛋鸡养殖场出现大量鼻炎症状的病鸡，主要表现为眼结膜发炎，眼睑肿胀、流泪，一侧或两则颜面肿胀，产蛋鸡产蛋量明显下降。为探索该病病因及制定有效防治措施，对病鸡样品进行了细菌学检验。通过细菌分离、PCR、平板凝集及血清型检测等方法，鉴定出4株副鸡禽杆菌，该分离株感染试验鸡能复制出与临床表现一致的病例。经实验室检验确认，引起该病的病原体是B型副鸡禽杆菌。

英文摘要：

In December 2012, a widespread disease of laying hens outbroke in some commercial layer farms in Anhui province, showing clinical symptoms such as nasal discharge, swelling of the face and epiphora, and obvious decrease in egg production. To identify the pathogen and establish control measures, samples were taken from sick birds and bacteriological examination was performed. Through bacterial isolation, bi- ochemical tests, PCR, plate agglutination test and serotyping, four isolates were identified. The SPF chickens infected with these isolates showed similar clinical signs as field birds. The laboratory examina- tion confirmed that the Avibacterium t~aragallinarum tvn~ 1~ w~ th~ ~,;~1,~;.ol