以耐铜性较强的烟草 W38和耐铜性较弱的本氏烟为对比实验材料,设置了0.0,0.5,1.0,2.0,4.0和8.0 mg/ L 共6个铜处理浓度,通过水培法探究了铜污染对烟草生长特性、根系活力、质膜透性及其抗氧化系统的影响.结果表明:随着铜处理浓度的递增,烟草的根长、株高、鲜质量、根系活力值及其过氧化氢酶、超氧化物歧化酶和过氧化物酶3种抗氧化酶的活性均先上升后下降,质膜透性值则先降低后升高,显示出低浓度的铜处理对烟草生长有促进作用,而高浓度的铜处理对其生长有抑制作用;其游离脯氨酸、丙二醛含量随着铜处理浓度的增加总体上呈现上升的趋势.
A solution culture experiment was conducted to study the effects of different copper concentration (0. 0,0. 5,1. 0,2. 0,4. 0,8. 0 mg / L)on the growth characteristic,root activity,membrane permeability and antioxidant system of two tobacco cultivars,Nicotiana W38( Cu resistance genotype)and Nicotiana benthamiana(Cu sensitive genotype). The results showed that:with increasing concentration of copper processing,root length,plant height,fresh weight,root activity,catalase(CAT),superoxide dismutase(SOD) and catalase(POD)of two tobacco cultivars were increased firstly and then decreased. The values of membrane permeability were decreased firstly and then increased. It showed that low concentrations of copper stimulated the growth of tobaccos,while high concentrations of copper inhibited tobaccos′ growth. With the in-crease of the concentration of copper processing,the content of proline(Pro)and malondialdehyde( MDA) showed a rising tendency.