中文摘要：

采用冻融法破碎发状念珠藻细胞，经过磷酸缓冲液提取、硫酸铵分步沉淀、离子交换层析和凝胶层析分离纯化发状念珠藻藻蓝蛋白，其纯度（A615／A280）达到5．321，纯化因子为7．623。纯化的藻蓝蛋白最大吸收峰为615nm，其室温荧光发射峰为646nm，SDS—PAGE电泳显示其d和13亚基的分子质量在14．4～18．4ku。稳定性实验表明，低温、避光、pH6～8以及低浓度的蔗糖等条件下发状念珠藻藻蓝蛋白是稳定的。实验结果将为开发利用发状念珠藻藻蓝蛋白提供参考。

英文摘要：

It＇s useful measure for the protection of the endangered natural resources to extract useful substances from artificial cultured species. In this study, C-phycocyanin was extracted from liquid-cultured Nostoc flagelliforme cells with repeated frost thawing followed by frozen ultrasonic method. The crude extract was successfully purified by procedure of ammonium sulfate precipitation, ion exchange chromatography with DEAE 650M column, and gel filtration chromatography with Sephadex G200 column. The purity ratio and purity factor of the purified C-phycocyanin reached 5. 321 and 7. 623 respectively after successive purification procedure. It has a maximum absorption at 615nm and a maximum fluorescence emission at 646nm. There are two bands appeared at about 15.7ku and 16.8 ku for SDS-PAGE, which are attributed to α and βsubunits of C-phycocyanin. The stability tests indicated that C-phyeoeyanin is stable in the range pH 6 to 8 with temperature below 30℃ in the dark or indoor light conditions. These results help the further study on exploitation of C-phycocyanin from N. flagelliforme cells.