中文摘要：

目的同时测定山楂叶提取物中2″-O-牡荆素葡萄糖苷（Glv）、2″-O-牡荆素鼠李糖苷（Rhv）、芦丁（Rut）、牡荆素（Vit）和金丝桃苷（Hps）的含量。方法采用HPLC法,色谱柱为DiamonsilTMC18柱（200 mm×4.6 mm,5μm）,流动相A为甲醇,B为乙腈-四氢呋喃（1：19）,C为0.5%冰醋酸,梯度洗脱。0-15 min,流动相A由1%-21%,B由19%-21%;15-17 min,A由21%-65%,B保持5%;17-20 min,A由65%-1%,B由5%-19%;流速1.0 ml·min^-1;检测波长360 nm;柱温30℃。结果Glv、Rhv、Rut、Vit、Hps的平均加样回收率分别为99.72%、100.27%、99.48%、99.31%和100.83%,其RSD分别为1.26%、1.69%、1.61%、1.70%和1.31%（n=6）。结论所建方法简便、快速、准确、可靠,可用于山楂叶提取物的质量控制。

英文摘要：

OBJECTIVE To establish an HPLC method to determine simultaneouly 2＂ - O - Glucosylvitexin （ GLV）, 2＂ - O - Rhamnosylvitexin （RHV）, Rutin （RUT）, Vitexin （VIT） and Hyperoside （HP） in Hawthorn leaves extract. METHODS The DiamonsilTM C18 column （200 mm × 4. 6 mm, 5 μm） was used at the temperature of 30 ℃ ; the mobile phase consisted of A： CH3OH, B ： CH3CN - THF（ 1 ： 19）, C ： 0.5% HAc ; gradient elution profile was 0 - 15 min,A： 1% - 21%, B ： 19% - 21% , 15 - 17 min, A ： 21% - 65%, Bkeep5%; 17 -20min, A ：65% -1%, B：5% -19%. Theflowratewas1.0ml·min^-1. The UV detector was set at 360 nm. RESULTS The average recoveries of GLV, RHV, RUT, VIT and HP were 99. 72%, 100.27%, 99.48% , 99.31% and 100.83% , with RSD of 1.26%, 1.69% , 1.61% , 1.70% and 1.31% （n = 6）, respectively. CONCLUSION The method is simple, rapid, accurate and reliable. It can be used to control quality of Hawthorn Leaves Extract and its preparations.