中文摘要：

高压快速冷冻（high-pressurefreezing,HPF）能使样品在高压下毫秒内从室温降到液氮温度,所有分子瞬间固定在原位,随后启用冷冻置换（freezesubstitution,FS）,在低温下缓慢固定的同时用有机溶剂替代样品中的水分,从而保存样品的细微结构接近自然状态.本文使用高压快速冷冻/冷冻置换方法对草地贪夜蛾细胞（s？细胞）和感染沙眼衣原体的小鼠成纤维细胞（McCoy细胞）进行电镜制样观察,同时对比传统化学固定两种细胞超微结构的差异.结果发现,利用HPF/FS技术,两种细胞中的膜结构、细胞骨架结构更能良好地保存,核膜、高尔基体和囊泡膜平滑完整,无皱缩或断裂,细胞形态更接近自然状态.

英文摘要：

High-pressure freezing （ HPF） can instantly fix all molecules in situ by decreasing the temperature from ambient temperature to about - 196 丈 in milliseconds. The following freeze substitution （ FS） can proceed with the dehydration at a low temperature. In this paper, the applications of HPF/FS in fixing the sf9 cells and the McCoy cells infected with Chlamydia trachomatis were reported. The results reveal that the cytoplasm, membrane structures, Golgi apparatus, vesicles and micro fiber structures in the cells with HPF/FS fixation are preserved better than those in the cells with chemical fixation.