研究了缘管浒苔新鲜组织、干粉末、培养水过滤液和水溶液抽提液对球等鞭金藻生长的克生作用。结果表明,缘管浒苔新鲜组织、干粉末和水溶性抽提液对球等鞭金藻的生长具有强烈的克生作用。当培养液中缘管浒苔新鲜组织、干粉末和水溶性抽提液的浓度为5.0g-wet/L、1.2g-dry/L和1.0g/L时,球等鞭金藻不能够维持正常的生长或全部死亡。在缘管浒苔培养水过滤液培养实验中,一次性添加缘管浒苔培养滤液,不能持久地抑制球等鞭金藻增殖,而半连续添加缘管浒苔培养滤液,其克生作用较强,说明克生物质的连续分泌是有效抑制球等鞭金藻生长的关键。经高温高压处理后的缘管浒苔培养滤液对球等鞭金藻无显著的抑制作用,说明缘管浒苔所分泌的克生物质在高温高压下不稳定和易分解。
The excessive growth of some species from green algae,such as,Ulva,Chaetomorpha and Cladophora,has been reported in the formation of macroalgal blooms or green-tide events in many parts of the world including Europe,North America,South America,Japan and Australia.In June 2008 the world’s largest green-tide ever covering about 600 km2 occurred along the coast of the Yellow Sea.Over one million tonnes of green algae were removed from the beach and coast,which caused huge economic loss to the local government.The cause,origin tracked and threatening to the marine environment of the green-tide and the possibility of recurrence have greatly concerned government officials and scientists.During the period of green algal bloom,macroalgae could inhibit the growth of phytoplankton,which could modify the trophic mode of marine ecosystem.Ulva linza was a popular species of green tides that occurred along the coast of the Yellow Sea.This paper studies the allelopathic effects of the macroalgae U.linza on the microalgae I.galbana,in order to provide some theoretical proof for evaluating the effects of green algal bloom on the marine environment.Allelopathic effects of fresh tissue,dry powder,macroalgae culture medium filtrate and aqueous extracts of U.linza on I.galbana were studied in the laboratory using coexistence culture system.Different initial inoculation concentrations(0,0.5,1.0,2.0 and 5.0 g-wet/L) of U.linza fresh tissue,(0,0.2,0.4,0.6 and 1.2 g-dry/L) of U.linza dry powder and (0,0.1,0.2,0.5 and 1.0 g/L) of U.linza aqueous extracts were used in coexistence culture systems.The results of the coexistence assays showed that the growth of I.galbana was strongly inhibited by fresh tissue,by dry powder and by aqueous extracts of U.linza.When the inoculation concentration of fresh tissue,dry powder and aqueous extracts was 5.0 g-wet/L,1.2 g-dry/L and 1.0 g/L,respectively,the cells of I.galbana could not grow normally or died completely within six to seven days.The effects of the U.linza culture medium filtrat