中文摘要：

通过同源克隆的方法从香樟Cinnamomum camphora中分离到一个编码延伸因子EF1a的cDNA序列片段。根据已经在GenBank中登录的其它物种的EF1a基因的保守序列设计一对兼并引物，通过反转录PCR技术获得一个1297 bp的基因片段。同源比对表明：CcEF1a与GenBank中注册的其它植物EF1a基因核苷酸序列的相似性均在80%以上，氨基酸序列的相似性高达96%以上。将所获得基因片段命名为CcEF1a并提交GenBank，登录号为KM086740。实时定量PCR结果显示，CcEF1a在低温胁迫下的叶片中表达量稳定，可以在实时定量PCR分析中作为内参基因。

英文摘要：

A cDNA fragment encoding a putative protein of elongat ion factor 1 alpha （EF1a） was ifrstly isolated from Cinnamomum camphora by using homology cloning, A pair of degenerate primers was designed based on the conserved sequences of other EF1a genes from the different species from GenBank, and a fragment of 1 297 bp was obtained through Reverse Transcription Polymerase Chain Reaction （RT-PCR） techniques. The results of homologous alignment show that CcEF1a shared over 80%nucleotide sequence identity and over 96%amino acid sequence identity with EF1a genes in other plants that had been submitted to GenBank. The cDNA fragment sequence was named CcEF1a and registered in GenBank with accession number KM086740. According to the results of real-time quantitative PCR, the mRNA level of CcEF1a in leaves under chilling stress was identical. It could be used as an interrnal control in real-time quantitative PCR.