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中文摘要：
为探究香樟Cc CBFb基因增强植株非生物胁迫抗性的功能,通过农杆菌介导法将该基因转入烟草中,经PCR和半定量RT-PCR技术鉴定阳性转基因株系后,对获得的T1代转基因无性系（T2和T4株系）以及野生型（WT）进行干旱（0、150、300和450 mmol·L^-1的甘露醇）、高盐（0、100、200和300 mmol·L^-1的Na Cl）、4℃低温、–4℃冰冻胁迫处理,结果显示：转基因烟草在干旱和高盐胁迫下,幼苗的存活率均高于野生型;经4℃低温处理后,转基因烟草植株的脯氨酸和可溶性糖含量均显著高于野生型植株,而丙二醛（MDA）含量均低于野生型植株;经–4℃的冰冻处理6 h后,野生型和转基因T4株系植株叶片均出现不同程度的萎蔫,而转基因T2株系植株未出现不良现象。由此可见,过表达CcCBFb基因不但能够增强烟草的抗旱和抗盐性,而且能够显著增强抗寒性。
英文摘要：
In order to explore the function of CcCBFb gene from Cinnamomum camphora, the effect of over-expression of this gene on abiotic stress resistance improvement is assessed by using transgenic tobacco. With the identification of positive transgenic tobacco by PCR and semi-quantitative RT-PCR technique, T1 clonal transgenic tobacco （Line 2 and Line 4） and wild type （WT） would be treated under the drought, salinity, low temperature and freezing stress. The results showed that survival rate of transgenic tobacco （Line 2 and Line 4） seedling was better to wild tobacco under drought, salinity stress. The proline and soluble sugar content of transgenic tobacco plants （Line 2 and Line 4） was higher than wild tobacco but the content of MDA was lower than wild tobacco under low temperature （4 ℃ ）treatment. WT and Line 4 had the different degree wilting but the Line 2 had a good growth status under the freezing treatment （ - 4 ℃ ） with 6 h. So, the overexpression of CcCBFb from camphor tree conferred tobacco plants enhanced the tolerance to cold, drought and salinity stress
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