中文摘要：

目的：观察丁酸钠（NaB）对体外培养的胃癌细胞系BGC823的生长抑制作用，并进一步探讨其作用机制。方法：应用MTT法检测细胞增殖活性；流式细胞仪检测细胞周期分布；Annexin V—FITC染色法检测细胞凋亡率。结果：不同浓度NaB处理胃癌细胞BGC82324～96h后．细胞增殖显著受到抑制，并呈浓度（P〈0．01）及时间（P〈0．05）依赖性；1．0，3．0，5．0mmol／LNaB处理72h后，BGC823细胞G0／G1期细胞数量显著增加，S期细胞数量显著降低（P〈0．01）；各浓度组细胞凋亡率均显著增加（P〈0．01）。结论：NaB对胃癌细胞系BGC823具有生长抑制作用，其机制与阻滞细胞周期及诱导凋亡有关。

英文摘要：

Objective： To investigate the effect of sodium butyrate （NaB）, one of histone deacetylase inhibitors （HDACI）, on the proliferation of human gastric cancer cell line BGC823 and its mechanism, Methods： BGC823 cells cultured in RPMI 1640 were treated with 0.5, 1. 0, 3. 0, and 5.0 mmol/L of NaB. MTT assay, flow cytometry, and Annexin V-FITC staining were performed to determine the cell proliferation activity, cell cycle, and apoptotic rate. Results： After BGC823 cells were treated with different concentrations of NaB for 24 to 96 hours, the cell proliferation activity was inhibited in a dose- and time-dependent manner （ P 〈 0.01, P 〈 0.05 ）. The number of cells in G0/G1 phase significantly increased and the number of cells in S phase significantly decreased after the cells were treated with 1.0, 3.0, and 5.0 mmol/L of NaB for 72 hours （both P 〈 0.01 ）. The apoptotic rate significantly increased in cells treated with different concentrations of NaB （all P 〈0.01 ）. Conclusion： NaB could inhibit the growth of human gastric cancer cell line BGC823 by blocking cell cycle and inducing apoptosis.