中文摘要：

房间趋于在本国的纸巾形成层次结构。在象癌症球状体和胚胎的身体那样的 vitro 的房间总数的形成提供一个唯一的工具在 vivo 学习机械性质和他们的对应物的生物行为 / 功能。在这份报纸，我们考察最先进的试验性的途径在 vitro 估计机械性质和房间总数的机械地导致的回答。这些途径根据装载形式被分类进五个范畴，包括 micropipette 渴望， centrifugation，装载的压缩，底层膨胀，和液体砍装载。我们讨论了每条途径，和潜在的生物医学的应用的优点和劣势。房间总数的机械行为的理解提供卓见给在生物功能的房间和正直之间的物理相互作用，它可以为象 atheromatosis 和癌症那样的疾病启用机械干预。

英文摘要：

Cells tend to form hierarchy structures in native tissues. Formation of cell aggregates in vitro such as cancer spheroids and embryonic bodies provides a unique means to study the mechanical properties and biological behaviors/functions of their counterparts in vivo. In this paper, we review state-of-the-art experimental approaches to assess the mechanical properties and mechanically-induced responses of cell aggregates in vitro. These approaches are classified into five categories according to loading modality, including micropipette aspiration, centrifugation, compression loading, substrate distention, and fluid shear loading. We discussed the advantages and disadvantages of each approach, and the potential biomedical applications. Understanding of the mechanical behavior of cell aggregates provides insights to physical interactions between cells and integrity of biological functions, which may enable mechanical intervention for diseases such as atheromatosis and cancer.