中文摘要：

目的观察不同暴露剂量和时间下，亚砷酸钠（NaAsO2）对体外培养的牛主动脉血管内皮细胞（BAEC）中血红素加氧酶-1（HO-1）的诱导作用。方法分别在0～15μmol／LNaAsO2暴露0～24h收集培养的BAEC细胞蛋白提取液中，用蛋白印迹（westernblot）法检测HO-1蛋白的诱导产生量。结果0～15μmol／LNaAsO2暴露能够显著诱导BAEC细胞HO-1蛋白产生增加，并且分别具有剂量-反应和时间-反应相关关系；2．5μmol／LNaAsO2暴露即可显著诱导产生HO-1蛋白。结论HO-1蛋白的诱导表明砷介导的一种细胞应激反应；HO-1蛋白可以作为无机砷暴露的敏感性生物标志物之一，有利于深入研究无机砷暴露的生物剂量反应。

英文摘要：

Objective To observe sodium arsenite （NaAsO2） -induced expression of heme oxygenase- 1 （HO- 1）in bovine aortic endothelial cells （BAEC）, Methods Cultured BAEC was exposed to NaASO2 （0- 15μmol/L） for 0- 24 h, respectively. Western blot was used to detect the induction of HO- 1 protein. Results 0 15 μmol/L NaAsO2 exposure induced the production of HO - 1 protein significantly, with dose - dependent and time - dependent relationship, Low dose of NaAsO2（2.5 μmol/L） could induce markedly the expression of HO - 1 protein, Conclusion HO - 1 induction could be a cellular stress response, and has potential for being used as a sensitive biomarker for inorganic arsenic exposure, which may be useful for further research of the biological response of arsenic.