采用氨基酸自动分析仪与电子单纤强力仪对棒络新妇(Nephila clavata)卵袋框丝与内层丝2种不同功能蜘蛛丝的氨基酸组成与力学性能进行测试研究,拟探明蛛丝组成、力学行为、性能与生物学功能之间的关系。结果表明,与卵袋框丝相比,卵袋内层丝的甘氨酸和脯氨酸含量较低,丝氨酸和极性氨基酸含量均较高,大侧链氨基酸(LC)与小侧链氨基酸(SC)的比值较高,初始模量较小,其拉伸曲线弹性区增加,屈服区很小,加强区平缓,延展性很好,拉伸行为重复性较好。
Electronic single-fiber tensile instron and amino acid auto-analyser were used to test the amino acid compositions and mechanical properties of two types of egg case silk of spider with distinct functions,i.e.,inner cover and scaffolding egg case silks in order to investigate the relationship between the structure,mechanical properties and bio-function of these unique materials.The results demonstrate that when compared with scaffolding egg case silk,the egg case inner silk has less Gly and Proline but higher Ser and polar amino acid.Its initial modulus is smaller and the value of LC:SC is higher.Its tensile curve displays an increase in the elastic region,small extent in the yield region,and buffering state in the reinforcing region,indicating very good tenacity and reproducibility of tensile behavior.