中文摘要：

为探讨原发性胆汁性肝硬化（PBC）患者血清抗线粒体抗体（AMA）亚型抗M2、M4、M9抗体的临床意义,采用间接免疫荧光法检测96例PBC患者血清AMA水平,采用酶免疫斑点法检测AMA-M2、M4、M9抗体水平,应用全自动生化分析仪检测ALT等生化指标。结果表明96例PBC患者中,荧光法AMA阳性率为84.4%,抗M2抗体为81.3%;斑点法抗M2抗体阳性率为72.9%,抗M4抗体为44.8%,抗M9抗体为18.8%,抗M2和M4抗体同时阳性阳性率为43.8%,抗M2和M9抗体同时阳性阳性率为16.7%,抗M2、M4和M9抗体同时阳性阳性率为13.5%,抗M4抗体阳性的PBC患者ALT、AST和IgM水平明显高于抗M4抗体阴性（P〈0.05）,抗M9抗体阳性的PBC患者ALT、AST和IgG水平明显低于抗M9抗体阴性（P〈0.05）。AMA-M2抗体的检测对PBC患者有诊断意义,抗M4抗体和抗M9抗体的检测对于PBC患者的病情判断有意义。

英文摘要：

To study the clinical sign M2,anti-M4 and anti-M9 in patients ificance of anti-mitochondrial antibody＇ s （AMA） subtypes antiwith primary biliary cirrhosis （PBC） , the serum levels AMA in 96 patients with PBC were detected by indirect immunofluoreseence and anti-M2, anti-M4 and anti-M9 levels by immunodotting, the ALT levels by automatic biochemistry analysator. The results showed that the positive ratio of AMA was 84.4% , the positive ratio of anti-M2 was 81.3% by indirect immunofluorescence. By immunodotting method the positive ratio of anti-M2 was 72.9% , the positive ratio of anti -M4 was 44.8% , the positive ratio of anti-M9 was 18.8% , the positive ratio of both anti-M2 and anti -M4 was 43.8% , the positive ratio of both anti-M2 and anti-M9 was 16.7% , and the positive ratio of anti-M2, anti-M4 and anti-M9 at the same time was 13.5%. The levels of the ALT, AST, IgM in patients with anti-M4 positive were higher than these with the anti-M4 negative （ P 〈 0.05 ）. The levels of the ALT, AST and IgG in patients with anti-M9 positive were lower than these with in anti-M9 negative （ P 〈 0.05 ）. The detection of anti-M2 antibody levels has clinical significance in the diagnosis of PBC. The detection of anti-M4 and anti-M9 levels have a important role to judge the pathogenetic conditions in patients with primary biliary cirrhosis.