中文摘要：

为深入研究百合鳞茎蔗糖代谢生理机制,以兰州百合（Lilium davidii var. unicolor）鳞茎外层鳞片为试材,研究了不同种类和pH值的缓冲液、反应时间和反应温度对可溶性酸性转化酶（Soluble acid invertase,SAI,EC3.2.1.26）活性的影响,建立了SAI活性的最佳检测体系。结果表明,最适提取缓冲液为pH8.0的Hepes-NaOH;最佳反应缓冲液为pH4.8的HAc-K3PO4,最适反应温度为40℃,最适反应时间为30min。

英文摘要：

In order to make a thorough study in sucrose-metabolizing mechanism of lily bulblet,the exterior scales of Lilium davidii var.unicolor bulb were used in present study to explore the effects of buffers with different sorts and pH values,reaction durations and reaction temperatures on SAI activity.Then SAI activity detection system was established.The results indicated that the optimal extraction and reaction buffers were Hepes-NaOH（pH 8.0）and HAc-K3PO4（pH 4.8）,respectively.And SAI has an optimum reaction duration and temperature of 30min and 40℃.