以东方百合‘Sorbonne’为试材,研究了GA3、IBA以及温度处理对鳞片扦插繁殖以及碳水化合物代谢的影响。结果表明:GA3处理可在较短时间内促进小鳞茎膨大,IBA处理使小鳞茎形成的时间延后,但可提高繁殖系数和整齐度。GA3、IBA分别结合5℃预处理可使小鳞茎质量显著增加,在较短时间内促进小鳞茎膨大;其中IBA100mg·L-1,1h结合5℃预处理的鳞片繁殖系数和小鳞茎整齐度依次高于25℃与15℃变温培养及25℃恒温培养;GA3100mg·L-1,2h结合5℃预处理获得的小鳞茎品质最佳。鳞片繁殖过程中,GA3、IBA结合各温度处理鳞片淀粉及总可溶性糖含量均呈下降趋势,尤其鳞片下部在25℃培养初期(20d)下降幅度最大;IBA促进淀粉降解,结合低温处理的母鳞片较无低温处理的总可溶性糖含量低,为小鳞茎的诱导和形成提供了营养;5℃低温预处理促进GA3处理母鳞片淀粉的降解。
Lilium Oriental hybrid'Sorbonne'were used to study the effects of GA3,IBA and temperature treatments on scales cutting propagation as well as the carbohydrate metabolism.The results showed that GA3 treatments could enlarge the bulblets in a short time,and IBA treatments made the formation of bulblets delay,but the propagation coefficient and the uniformity of bulblets could be increased.GA3 and IBA combined with 5 ℃ pretreatment separately could increase the mass of bulblets significantly,and could promote bulblets to enlarge in a short period;IBA 100 mg · L-1,1 h combined with 5 ℃ pretreatment made the propagation coefficient and the uniformity of bulblets higher than 25 ℃/15 ℃ variable temperature culture as well as 25 ℃ constant temperature culture;GA3 100 mg · L-1,2 h combined with 5 ℃ pretreatment could obtain the best quality of bulblets.During scales reproduction,GA3,IBA with different temperature treatments made the content of starch and total soluble sugar decline in scales,especially which in the base of scale decreased sharply in the early stage at 25 ℃(20 d);IBA promoted the degradation of starch,which provided the nutrition for the induction and formation of bulblets.The content of total soluble sugar in mother scales was lower under IBA combined with low temperature treatments than those with non-cold treatments.5 ℃ pretreatment promoted the degradation of starch in mother scales with GA3 treatment.