中文摘要：

将从东亚钳蝎中克隆到的兴奋型昆虫毒素基因（BmK IT）同源重组到苜蓿银纹夜蛾核型多角体病毒（AcM-NPV）基因组中,得到重组病毒AcMNPV-BmK IT,抗虫试验表明重组杆状病毒的杀虫活性明显优于野生型病毒,但AcMNPV介导的BmK IT的抗虫分子机制尚未阐明.本试验从草地贪夜蛾Sf9细胞中克隆获得了凋亡相关基因Sfp53,制备了抗体,分析了AcMNPV-BmK IT对Sfp53表达的影响,结果表明被重组病毒感染的细胞所表达的Sfp53时间与表达量与野生型相比都有所提前和提高,说明重组病毒可加速细胞的凋亡;同时通过半定量PCR分析了AcMNPV-BmK IT感染Sf9细胞时病毒抗凋亡基因iap2的表达,结果表明重组型病毒抗凋亡基因iap2表达量减少.以上结果在细胞分子水平上解释了AcMNPV-BmK IT杀虫活性提高的原因.

英文摘要：

We engineered an insect toxin gene from Buthus martensii Karsch （BmK IT gene） into the genome of AcMNPV （Autographa californica multicapsid nucleopolyhedrovirus） in our previous study. The bioassay data indicated that the recombinant baculovirus AcMNPV-BmK IT significantly enhanced the insecticidal activity. However, nothing is known about the apoptosis mechanism of AcMNPV-BmK IT. In this study, Western blot and semi-quantitative PCR were used to analyze the effects of AcMNPV-BmK IT on the expression of apoptosis-related genes, Sfp53 and iap2. The results showed that AcMNPV-BmK IT increased the expression of Sfp53 and inhibited the expression of iap2, which explained the enhanced insecticidal activity of AcMNPV-BmK IT in cellular and molecular levels.