中文摘要：

以腺病毒为载体介导东亚钳蝎氯离子通道神经毒素（BmKCT）感染人脑神经胶质瘤细胞株U251，研究其对细胞迁移及凋亡的影响，并探讨可能的作用机制．首先以含BmKCT基因的腺病毒Ad_BmKCT及对应的腺病毒空载体转染U251细胞筛选出最佳感染浓度，随后利用BoydenChamber实验和划痕实验检测了BmKCT对U251细胞迁移能力的影响，MTT检测了细胞存活能力，用AnnexinV-Cy5和PI双标记流式检测分析细胞凋亡情况，并通过Western—blot检测凋亡相关蛋白分析可能的凋亡机制．结果显示，U251细胞感染Ad-BmKCT48h后，迁移能力受到明显抑制；细胞变圆漂浮，Western—blot检测发现细胞色素C开始释放，caspase-3表达上调．

英文摘要：

Chlorotoxin （CTX） was reported to inhibit glioma invasion and metastasis. BmK CT, a venom peptide with 68% homology to chlorotoxin,was expressed by using adenovirus vector to study its effects on the migration and apoptosis of human U251 glioma cell. Firstly,Boyden Chamber and wound healing as- says showed that expression bf BmK CT restrained migration in U251 cells. Secondly,cells infected by Ad- BmK CT showed remarkably reduced proliferation rate in MTT assay, which might due to the promotion of apoptosis in BmK CT positive cells. Finally, BmK CT facilitated cytochrome c release and upregulated caspase-3,which might account for the increased apoptosis rates in BmK CT positive cells. This study pro- vided an experimental evidence that Ad-BmK CT might be a potential succedaneum of CTX for the treat- ment of glioma.