采用盆栽试验,设置4个旱涝交替胁迫处理(旱-轻涝-旱连续胁迫,其中分蘖期轻涝为保持水深10 cm,拔节期轻涝为保持水深15 cm):分蘖期轻旱(T-LD)、分蘖期重旱(T-HD)、拔节期轻旱(S-LD)和拔节期重旱(S-HD),并以常规灌溉(CK)为对照,研究了旱涝交替胁迫条件下水稻分蘖期和拔节期荧光特性。结果表明,分蘖期和拔节期旱涝交替胁迫均对叶绿素荧光参数产生显著影响。分蘖期第一次旱后,T-HD处理最大荧光值(Fm)比T-LD处理高16.29%(p〈0.05),而T-LD处理Fm则比CK降低3.44%;旱涝急转后,旱胁迫处理初始荧光值(Fo)略微低于CK,T-LD处理Fm比CK高1.98%,而T-HD处理则比CK低4.73%;第二次旱后,T-LD、T-HD处理Fo均低于CK,但PSⅡ最大光化学量子产量(Fv/Fm)比CK高。拔节期第一次旱后,S-LD和S-HD处理Fo高于CK,但Fm和Fv/Fm均比CK低;旱涝急转后,S-HD处理Fv/Fm最低;第二次旱后,S-LD和S-HD处理Fo分别比CK低4.40%和3.30%,而Fm则分别比CK高出17.02%和23.87%,且Fv/Fm显著高于CK(p〈0.01),荧光反应得到增强。复水至拔节期,分蘖期处理叶绿素Fo呈现先降低后升高的趋势,而Fm则上下波动,随后逐渐超过CK,且Fv/Fm低于CK(除9月6日外),说明PSⅡ反应中心的活性受到持续抑制;而在灌浆期后,各处理荧光参数差异较小。
Pot experiments were performed to research the effects of alternative stresses of drought and waterlog- ging (ASDW) at tillering and jointing stages on fluorescence characteristics of rice chlorophyll, and four treat- ments were set up (light drought in tillering stage (T-LD), severe drought in tillering stage (T-HD), light drought in jointing stage (S-LD) and severe drought in jointing stage (S-HD) using regular irrigation (CK) as the control (drought-light waterlogging-drought continuous stress, the water depths of waterlogging were 10 cm and 15 cm at tillering stage and jointing stage). The results showed that alternative stresses of drought and waterlogging at tillering and jointing stages both had significant effects on chlorophyll fluorescence parameters. After the first time drought at tillering stage, the maximum fluorescence value (Fro) of T-HD treatment was 16.29% higher than that of T-LD treatment (p 〈 0.05), while Fm of T-HD treatment decreased by 3.44% compared to CK; after the sharp turn from drought to flood, initial fluorescence value (Fo) of drought stress treatments were slightly lower than that of CK, Fm of T-LD treatment was 1.98% higher than that of CK, while Fm of T-HD treatment was 4.73% lower than that of CK; after the second drought, Fo of T-LD and T-HD treatments were lower than that of CK, but the maximal quantum chemical yield of PS II (Fv/Fm) was higher than that of CK. After the first time drought at jointing stage, Fo of S-LD and S-HD treatments were higher than those of CK, but Fm and Fv/Fm were lower than those of CK; after the sharp turn from drought to flood, Fv/Fm of S-HD treatment was the mini- mum; after the second drought, Fo of S-LD and S-HD treatments were 4.40% and 3.30% lower than that of CK, but of which Fm were 17.02% and 23.87% higher than those of CK, respectively, and Fv/Fm were significantly higher than CK (p 〈 0.01), so it could be conducted that the fluorescence reaction was enhanced. As rewatering to jointing