在平板式生物膜反应器内,研究波长范围为420~590mm的3种不同单色光源对高效光合产氢细菌生物膜生长特性的影响进行实验,讨论了不同光照波长对沼泽红假单胞茵生物膜表面覆盖率、膜厚、干重和密度的影响。实验结果表明,不同的光照波长对生物膜生长速率及结构具有重要影响,在相同的时间间隔内,生物膜表面覆盖率随着光照波长的增加而增加,而光照波长对生物膜干重影响很小,在实验运行4d后,生物膜厚度随着波长的增加而减小;光照波长越长,生物膜密度越高,这是由于较短的光照波长抑制了细菌的分裂及胞外高聚物的生成。
In a plate-type bioreactor for biofilm development, the experiments for the growth characteristic of high-efficiency hydrogen-production photosynthetic bacterial biofilm was undertaken under three different monochromatic light source with light wavelengths varied from 420 nm to 590 nm. The effect of light wavelength on the parameters of Rhodoseudomonas palustris biofilm is discussed, such as the surface coverage rate, thickness, dry weight, and density. The light wavelength has significant influences on the growth rate and structure of biofilm. Increased wavelength results in a higher surface coverage rate. While the dry weight of biofilm keeps almost constant at the same period. After 4 days, the thickness of biofilm decreases with the increase in light wavelength while longer light wavelength leads to higher density of biofilm, which results from the cell division and production of EPS by short light wave.