中文摘要：

目的：检测富血小板血浆（platelet-rich plasma,PRP）作为人牙髓细胞（dental pulp cells,DPCs）组织工程学支架的生物相容性,并探讨PRP促进DPCs矿化的作用。方法：应用经典的两步离心法制取PRP;分离培养DPCs,并经细胞角蛋白、波形蛋白染色鉴定细胞来源。实验分为4组,将PRP和第4代DPCs复合激活后,植入8只5周龄雌性裸鼠背部皮下作为实验组,将单独植入DPCs或PRP做为对照组,将单纯手术组做为空白对照。分别于植入术后4周、8周处死动物,将生成物制成组织学切片进行HE染色和免疫组织化学染色。结果：植入4周、8周后,DPCs复合PRP组在裸鼠皮下均可见白色生成物,单独植入DPCs或PRP组未见生成物。DPCs复合PRP组HE染色可见矿化组织形成,免疫组织化学染色显示骨桥蛋白（OPN）、骨钙素（OC）和Ⅰ型胶原（COLⅠ）阳性表达。结论：富血小板血浆与人牙髓细胞有良好生物相容性,并对人牙髓细胞有诱导矿化作用,提示富血小板血浆可以作为盖髓治疗的支架应用。

英文摘要：

Objective：To investigate the biocompatibility of human platelet-rich plasma（PRP） and human dental pulp cells（DPCs）,and the effect of human platelet-rich plasma on the mineralization of human dental pulp cells in vivo.Methods： DPCs were isolated from healthy dental pulp,and identified by immunostaining of vimentin and cytokeratin.PRP was obtained from healthy volunteer donors by traditional two-step centrifugation.The forth passage of DPCs and PRP were mixed well and activated,and then transplanted subcutaneously in 5-week female nude mice.The groups which were implanted with PRP alone or DPCs alone were used as controls.The animals were sacrificed after 4 weeks and 8 weeks post-transplantation,and the histological and immunohistostaining examinations were used to evaluate the effect of PRP on the mineralization of DPCs.Results：Immunostaining showed that DPCs were positive for vimentin and negative for cytokeratin.In vivo assay showed that the newly formed mineralized tissues were only found in PRP combined with DPCs group after 4 weeks and 8 weeks,while newly formed tissues were not observed in PRP alone or DPCs alone groups.HE staining showed the mineralized tissues were found in PRP＋DPCs samples.Immunohistochemistry staining showed these mineralized tissues were positive for osteopontin（OPN）,osteocalcin（OC） and collagen Ⅰ（COL Ⅰ）.Conclusion： PRP had good biocompatibility with DPCs,and could induce the mineralization of DPCs.The study suggests that platelet-rich plasma can be used as a scaffold for pulp capping.