中文摘要：

目的为构建羊传染性脓疱病毒（ORFV）B2L基因真核表达质粒并验证B2L基因在BHK-21细胞中的表达及表达的稳定性。方法以ORFV主要免疫原性基因B2L为目标，以pVAX1为表达载体，构建重组真核表达质粒pVAX1-B2L，鉴定正确后转染仓鼠肾细胞（BHK-21），通过间接免疫荧光试验（IFA）验证B2L基因表达。结果扩增出目的片段经序列测定和分析证明为ORFVB2L基因，经双酶切鉴定和序列测定分析证明了pVAX1-B2L质粒的正确性，IFA证实目的基因在BHK-21细胞中能够瞬时表达。结论为进一步研制基于羊口疮病毒B2L基因的DNA疫苗奠定了基础。

英文摘要：

The objective is to construct DNA vaccine carrier for ORFV B2L gene and confirm its expression in BHK-21 cells. In this study, B2L gene and pVAX1 expression vector were used to construct recombinant eukaryotic expression plasmid pVAX1-B2L. BHK-21 cells were transfected with pVAX1-B2L plasmid using liposome, and the expression of B2L gene in BHK-21 cells was proved by indirect immunofluorescence assay （IFA）. The results indicated that B2L gene was amplified and confirmed by sequencing and analysis l recombinant plasmid pVAX1-B2L was validated by sequencing and two enzymes diges- tion; instantaneous expression of B2L gene was verified with IFA. This paper would lay the foundation for further research to- ward DNA vaccine based on B2L gene from ORFV.