中文摘要：

对6株细菌和硝酸氧化的山西临汾褐煤进行菌-煤匹配降解实验,筛选出降解优势菌恶臭假单胞菌.在单因素试验的基础上,通过正交试验优化了恶臭假单胞菌降解临汾褐煤的工艺条件,得出的影响权重为：煤样粒度〉煤浆质量浓度〉培养时间〉接种量.较优降解工艺条件为：煤样粒度〈75μm,煤浆质量浓度0.200 0g/10mL,培养时间14d,接种量1.0mL/10mL.此条件下的降解转化率达到46.81%.对降解后的煤残渣和降解产物进行红外检测,结果表明：煤残渣在氧化煤既有波数处的吸收大部分已消失或者变弱,说明煤中的有机大分子已被恶臭假单胞菌降解成小分子,包含在降解后的黑色油状滤液中.滤液分级萃取物的GC-MS总离子流色谱证明了降解液中含有丰富的小分子有机物,且大多为脂肪烃、酯、羧酸及芳香族化合物.

英文摘要：

The degradation matching experiments of Shanxi Linfen lignite with six kinds of bacteria had been investigated,showing that Pseudomonas putidais the most effective degrading bacterium.Based on single factor experiments,the degrading process conditions of Pseudomonas putida and Linfen lignite were optimized by orthogonal experiments.The results show that the effect-weight order of the factors for degrading rate is coal particle size 〉coal slurry mass concentration〉 incubation time 〉inoculum size,the best degrading technological conditions are coal particle size 〈75μm,coal slurry mass concentration of 0.200 0g/10 mL,incubation time of 14 d,inoculum size of 1.0 mL/10 mL.Under this technological conditions,the conversion rate reaches 46.81%.The coal residue and degrading product were tested,the infrared analysis shows that the absorption of coal residue at the wavenumber of oxidized coal has been disappeared or weakened,which further indicates that organic macromolecular in coal has been degraded by Pseudomonas putida into micromolecule containing in the black oily filtrate derived from the degradation.The total ion chromatogram of fractional extraction product of degradation filtrate demonstrates that it contains abundant small molecule organics,which mostly include aliphatic hydrocarbons,esters,carboxylic acids and aromatic compounds.