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Small RNA existed in commercial reverse transcriptase: primary evidence of functional small RNAs
  • ISSN号:0253-2654
  • 期刊名称:《微生物学通报》
  • 时间:0
  • 分类:Q55[生物学—生物化学] S511[农业科学—作物学]
  • 作者机构:[1]Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China
  • 相关基金:We would like to thank Mr. Chao Gao for his technical support. This work was supported by grants from the National Natural Science Foundation of China (Grant Nos. 31100777, 30988003 and 90608010) and the Natural Science Foundation of Jiangsu Province (BK2011013). Jie Xu, Xi Chert, Donghai Li, Qun Chen, Zhen Zhou, Dongxia Hou, Jin Wang, Qipeng Zhang, Ke Zen and Chen-Yu Zhang declare that they have no conflict of interest. All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2000. Informed consent was obtained from all patients for being included in the study. All institutional and national guidelines for the care and use of laboratory animals were followed.
中文摘要:

MicroRNAs (miRNAs ) 是小 RNA 分子(约 22 核苷酸渴望) 参加基因表示的 post-transcriptional 规定(陈和 Rajewsky, 2007 ) 。自从他们的发现,千这些 RNA 被报导了。这些小分子被显示了在大量生物、病理学的过程起一个重要规章的作用(Brennecke 等, 2003;Cuellar 和 McManus, 2005;Lim 等, 2005 ) 。最近, miRNAs 在浆液,血浆,尿和其它被发现了身体液体(陈等, 2008;威伯等, 2010 ) 。有趣地,这些细胞外的 miRNAs 的表示侧面与各种各样的疾病被相关;他们可以在诊断并且监视人的疾病被用作 biomarkers (陆等, 2005 ) 。

英文摘要:

SMALL RNA IN REVERSE TRANSCRIPTASE: TOO SMALL TO BE FOUND Significant background in no-template or no-primer RT-PCR is frequently noted. However, the underlying mechanism is still unclear. In this issue, Jie Xu and colleagues reveal that the com- mercial reverse transcriptases contain small RNAs. In AMV reverse transcriptase, they detected microRNAs derived from the virus host chick or other birds. This surprising finding at least partially explains the high background in some AMV reverse transcriptases based RT-PCR.

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期刊信息
  • 《微生物学通报》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中国科学院微生物研究所 中国微生物学会
  • 主编:赫荣乔
  • 地址:北京市朝阳区北辰西路一号院3号中国科学院微生物研究所内B401室
  • 邮编:100101
  • 邮箱:xuj@sun.im.ac.cn
  • 电话:010-64807511
  • 国际标准刊号:ISSN:0253-2654
  • 国内统一刊号:ISSN:11-1996/Q
  • 邮发代号:2-817
  • 获奖情况:
  • 1992年中国科学技术协会优秀学术期刊三等奖,1992年国家科委中共中央宣传部新闻出版署“全国优...,2000年中国科学院优秀期刊三等奖,中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),波兰哥白尼索引,美国剑桥科学文摘,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:29487