中文摘要：

狼毒是蒙医临床上常用的蒙药材之一,具有较强的抗癌作用,但狼毒具有很强的毒性作用,为降低狼毒毒性,同时保证良好的药效作用,更好的应用于临床,用斑马鱼初期胚胎作为实验模型,探讨了不同炮制方法对狼毒毒性影响.分别用酒精炮制(A)、奶炮制(M1)、酸奶炮制(M2)、醋炮制(S)和诃子炮制(K)狼毒,对斑马鱼胚胎进行染毒.结果显示醋炮制用50ug/ml和500ug/ml的浓度给2hpf斑马鱼胚胎染毒,发育到48hpf的死亡率分别是32.5%和55%,其他各种处理组死亡率没有明显增高.各种炮制方法染毒的斑马鱼胚胎,在尾鳍部位都出现锯齿状缺失,通过TUNEL染色检测确认为大量细胞凋亡.实验表明斑马鱼胚胎可以作为狼毒毒性评价与药物机理研究的动物模型.

英文摘要：

As a perennial herb used in Mongolian medicine, Sellera chamaejasme has strong anticancer effects but is also highly toxic. To reduce its toxicity, the effects of alcohol (A), milk(M1), yogurt(M2), vinegar(S) and myrobalan(K) processing on zebra embryos were compared. Vinegar extraction was the most toxic. Even at 500 ug/ml and 50 ug/ml, 55%and 32.5%of zebrafish embryos were killed within 48 h. The mortality rate remains almost the same by other ex-tracting methods. Exposure to all the extractions results in rugged appearance of caudal fins and cells of that region shows positive staining with TUNEL. The number of apoptotic cells of zebrafish embryo tail fins increased from 3.3 in the control group to 9.6-18.3 in the exposure group. A further study will be made on proliferation and apoptosis re-lated gene and protein expression.