中文摘要：

采用直链淀粉-三（3，5-二甲基苯基氨基甲酸酯）键合型手性固定相（Chiralpak IA柱）和涂敷型手性固定相（ChiralpakAD—H柱）对6个O，O’-二烷基-α-（氟代苯乙基）氨基-3，4，5-三甲氧基苯基甲基膦酸酯化合物进行了直接手性拆分研究，在正己影异丙醇、正己影乙醇两种不同流动相条件下，6个化合物均能在AD—H柱上实现基线分离，AD—H柱的手性分离效果优于IA柱。并考察了AD—H柱在以正己影乙醇作流动相时，不同温度及乙醇浓度对手性分离的影响。结果表明，在10—40℃范围内随着温度的降低，化合物的分离因子、分离度均增大，在乙醇体积分数为5％-15％范围内，随乙醇浓度的降低，化合物的分离度增大，分离因子基本保持不变。表明AD-H柱可推荐用于此类化合物的半制备手性分离。

英文摘要：

The direct HPLC enantioseparation of six O, O＇-dialkyl-α-（ fluorinated phenylethyl ） amino- 3,4,5-trirnethoxybenzyl methyl phosphonates on coated and immobilized amylose Iris-（ 3, 5- dimethyphenylcarbamate） chiral stationary phases （Chiralpak AD-H and Chiralpak IA） was presented. In n-hexane/2-isopropanol and n-hexane/ethanol mixtures, all studied compounds can be baseline separated on AD-H column, AD-H column exhibits better separation effect for all compounds than IA column. The effect of temperature and ethanol organic modifier concentration on the resolution was investigated on AD-H column. The results showed in the range of 10 - 40℃, resolution factors and sepatation factors increased with decreasing temperature; in the range of 5% - 15% ethanol in n- hexane, resolution factors increase with decreasing solvent polarity and separation factors were relatively stable. AD-H was recommended to carry out chiral semi-preparative separation of all compounds.