目的:关于天麻中8种有机氯农药的毛细管气相色谱测定方法的研究。方法:样品经有机溶剂超声波提取,用HP-5石英柱程序升温技术分离,电子捕获检测器检测,外标法计算含量。结果:样品的平均回收率为80%.95.6%;RSD为4.9%~6.0%;被测样品均有不同程度的农药残留。结论:本方法快速、简便、准确,具有良好的通用性,可用于天麻中8种有机氯农药残留量的测定。
Objective :To determine 8 organochloride pesticides in Gastrodia elata B1. by capillary gas chromatography with electron capture detector( CGC -ECD). Method:Samples were extracted with petroleum ether using supersonic fluid extraction. The column was HP - 5 ( 30 m × 0. 32 mm × 0. 25 μm ). The injector temperature was 250 ℃ and the detector temperature was 320 ℃. The column temperature program was initial temperature 60 ℃, with the increasing rate of 20 ℃ · min^ -1 to 160 ℃ ( hold 5 min), 10 ℃ · min^ -1 to 260 ℃ ( hold 2 min). The pesticide residues were calculated by external standard method. Results:The average recoveries and RSD ranged from 80. 0% -95. 6% and 4. 9% -6. 0% respectively,at three spiked mixed organochlorine pesticide levels. Conclusion:The method is easy in operation and has good separation and repeatability.