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中文摘要：
目的分析机采血小板在常温保存过程中凋亡相关miRNA的表达改变。方法应用miRNA微孔板芯片技术研究常温保存机采血小板在不同保存时间凋亡相关miRNA的差异表达谱，采用生物信息学软件预测发生差异表达的miRNA可能的靶基因及相关生物学功能。结果与新鲜血小板相比，常温保存7d的血小板，有7种miRNA发生了显著变化。其中miR-16、Let-7b、miR-26和Let-7c的表达上调，miR-7、miR．145和miR-197的表达下调。结论筛选出的差异表达miRNA可能参与了血小板的保存损伤过程。
英文摘要：
Objective To explore the variant expression profile of apoptosis-related microRNA （miRNA） in apheresis platelets during room temperature storage. Methods miRNA plate array was used to investigate the apoptosis-associated miRNA profile of apheresis platelets after different storage periods at room temperature. Various biological information soft- ware and databases were utilized to predict target genes and molecular functions of the selected miRNA. Results After room temperature storage for 7 days, as compared with fresh platelets, seven miRNA demonstrated high expression such as miR- 16, Let-7b, miR-26, and Let-7c, which were significantly upregulated, and miR-7, miR-145 and miR-197, which were downregulated. Conclusion The differentially expressed miRNA in this study may play an important role in the development of platelet storage lesion.
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