中文摘要：

通过比较丝线、羊肠线2种不同材料和不同结扎道数对大鼠CCI疼痛模型痛阈值的影响，优化筛选出更易在实验过程中普及的技术方法．将42只SD雄性大鼠随机分成7组，包括结扎丝线4道、3道与2道3个组；结扎羊肠线4道、3道与2道3个组和1个假手术组，每组6只大鼠．铬制羊肠线和丝线分别结扎大鼠左侧坐骨神经2道、3道、4道；假手术组只暴露左侧坐骨神经，不进行结扎处理．建模成功后测量各组大鼠的机械性缩足反应阈值（MWT），发现丝线3道、4道组和羊肠线2道、3道、4道组与假手术组和自身基础的MWT有极显著差异（P〈0．01）；假手术对照组、丝线2道组与各组自身基础的MWT之间没有差异（P〉0．05），结扎手术前后的MWT之间差异显著，结扎后第7天、14天和21天的MWT之间没有差异．羊肠线3组之间没有显著性差异（P〉0．05）．由此可得出结论：羊肠线2道、3道、4道组，以及丝线3道、4道组均能产生稳定的神经痛，根据MWT的数值大小和是否出现自噬现象可认为，以羊肠线结扎2道组最好，不但MWT的数值小，且操作简便又节省时间，可作为大鼠CCI建模的首选方法．

英文摘要：

In our report, the mechanical pain threshold （MWT） of CCI pain models made with different thread materials and number of ligation rings were compared, and the easier methods of CCI （ chronic constriction nerve injury model） were optimized and screened. Forty-two male SD rats were randomly divided into 7 groups, which were three CCI groups which is 4, 3 and 2 ring silk thread ligation, respectively, three CCI groups which is 4, 3 and 2 ring chromic gut thread ligation, respectively, sciatic nerves in rats of 6 CCI groups were ligated with and one group of sham-operation group （n = 6） ; the left chromic gut and silk thread, respectively, the left sciatic nerves in rats of sham-operation group were exposed without ligation. After the model was established, the MWTs were determined. The results indicated that there are significant differences between two CCI groups of 4, 3 ring silk thread ligation, three CCI groups of 4, 3 and 2 ring chromic gut thread ligation, and one group of sham-op-eration group and their base threshold （P 〈 0.01 ） ; there are no significant differences between sham-operation group, base threshold of each group and CCI group of 2 ring silk thread ligation （ P 〉 0.05 ） ; the MWTs before ligation surgery in CCI groups were different from their MWTs after surgery; the MWTs in CCI groups keep stable on 7, 14 and 21 days after surgery; there are no significant differences between the three chromic gut thread groups （ P 〉 0.05 ）. The three CCI groups of 4, 5 and 2 ring chromic gut thread ligation and two CCI groups of 4, 3 ring silk thread ligation produced stable neuralgia. According to MWT value and whether there were self-bites, the CCI group of 2 ring chromic gut thread ligation was the best model, which was the easiest operation and had low MWT value.