中文摘要：

建立肌肉型乙酰胆碱受体（胎儿型α1β1δγ和成年型α1β1δε）在非洲爪蟾卵母细胞中的表达模型,用于以这两种受体为靶点的药物筛选。将肌肉型乙酰胆碱受体的各个亚基基因在体外转录获得各自的cRNA,显微注射到非洲爪蟾卵母细胞中,利用双电极电压钳技术检测其表达情况,并确定不同浓度的乙酰胆碱对胎儿型α1β1δγ和成年型α1β1δε受体电流表达情况的影响。在卵母细胞中成功表达两种肌肉型乙酰胆碱受体;当乙酰胆碱浓度为1μmol/L时,α1β1δγ和α1β1δε受体电流很小,不能用于后续的检测试验,当浓度为5μmol/L时,完全激活α1β1δγ受体的开放电流达到最大6025 nA,当浓度为20μmol/L时,完全激活α1β1δε受体的开放电流达到最大2 742 nA,只注射ddH2O的对照组细胞则没有任何电流产生。该受体模型的成功建立,为筛选作用于肌肉型乙酰胆碱受体的药物提供了实验模型。

英文摘要：

To establish the expression models of fetal（α1β1δγ） and adult（α1β1δε） muscle acetylcholine receptors（nAChRs） in Xenopus laevis oocytes for drug screening based on these two molecular targets.Each subunit cRNA of the muscle nAChRs was obtained by in vitro transcription,which was microinjected in Xenopus laevis oocytes for expression.The oocytes were incubated in ND96 buffer at 17 ℃ over 24 h after microinjection.Two-microelectrode voltage clamp（TEVC） was used to detect the acetylcholine gated current of α1β1δγ and α1β1δε nAChRs.The α1β1δγ and α1β1δε muscle nAChRs were expressed in Xenopus laevis oocytes successfully.When the ACh concentration of 1 μmol / L,α1β1δγ and α1β1δε receptor currents are small,and can not be used for subsequent testing.The concentration of Ach 5 μmol / L,fully activated receptor α1β1δγ-current reached the maximum 6 025 nA.The Ach concentration of 20 μmol / L,fully activated receptor α1β1δγ-current reached the maximum 2 742 nA,while no current was recorded ddH2O injected oocytes.The establishment of the receptor expression offered the experimental model to screen drugs targeting α1β1δγ and α1β1δε nAChRs.