中文摘要：

【目的】明确温度对西伯利亚蝗Gomphocerus sibiricus成虫呼吸代谢关键酶活性的影响。【方法】18~42℃范围内,以3℃为梯度,将西伯利亚蝗成虫置于光照培养箱黑暗条件下处理4 h后,利用生化方法测定3-磷酸甘油醛脱氢酶（GAPDH）、3-磷酸甘油脱氢酶（GPDH）、3-羟酰辅酶A脱氢酶（HOAD）、柠檬酸合成酶（CS）和乳酸脱氢酶（LDH）5种呼吸代谢关键酶活性。【结果】随温度升高,西伯利亚蝗成虫体内5种呼吸代谢关键酶活性先增后减,相同温度下雄虫的酶活性均高于雌虫;18℃下雌雄成虫的5种关键代谢酶活性最低,39℃下雌虫的GAPDH和CS活性最高,雄虫CS活性在36℃时最高;不同处理温度下,雌虫之间、雄虫之间关键代谢酶活性差异均显著（P〈0.05）。不同处理温度下雌雄虫GAPDH与HOAD活性比值均大于或接近于1.0,说明低温阶段西伯利亚蝗呼吸代谢消耗以糖类为主,高温胁迫下糖类和脂类同时被利用。【结论】温度对西伯利亚蝗成虫体内呼吸代谢关键酶活性有显著影响,高温胁迫下西伯利亚蝗可通过调整呼吸代谢强度和改变消耗底物种类提高自身的适应能力。

英文摘要：

【Aim】This study aims to clarify the effects of temperature on the activities of key enzymes related to respiratory metabolism in adults of the Siberian grasshopper,Gomphocerus sibiricus.【Methods】By using biochemical methods,the activities of five enzymes,i.e,glyceraldehyde-phosphate（GAPDH）,glycerol-3-phosphate（GPDH）,lactate dehydrogenase（LDH）,3-hydroxyacyl-Co A dehydrogenase（HOAD） and citrate synthase（CS）,in G.sibiricus adults were measured after they were treated in Light Emitting Feed Box for 4 h under darkness within a temperature gradient of 18-42℃ in a 3℃ interval.【Results】The activities of five key enzymes related to respiratory metabolism in G.sibiricus adults increased firstly and then decreased,with the values of males higher than those of females at the same temperature.The enzyme activities in both males and females were the minimum at 18℃.The activitiesof GAPDH and CS of females reached the maximum at 39℃,while the CS activity in males reached the peak at 36℃.At different temperatures,the enzyme activities between males and females were significantly different（P 〈 0.05） except the CS activity,and the ratios of GAPDH activity to HOAD activity were more than or close to 1.0,suggesting that carbohydrates are consumed for respiration at lower temperature,while lipid and carbohydrates are used as energy substances at higher temperature.【Conclusion】Temperature has significant effects on the activities of key enzymes related to respiratory metabolism in G.sibiricus adults,which change the intensity of respiratory metabolism and energy substrates for improving the capacity of adapting to high temperature stress.