中文摘要：

目的：探讨小型猪肝硬化模型制备过程中肝脏多层螺旋CT血流灌注的变化。方法：同种系巴马小型猪45头,随机分为实验组（40头）和对照组（5头）,实验组采用四氯化碳复合因素法造模,对照组正常喂养,两组动物分别在4周、8周、12周、16周、20周末进行多层螺旋CT血流灌注扫描及肝穿刺活检,并分析肝动脉灌注量（HAP）、门脉灌注量（PVP）、总肝灌注量（THBF）及肝动脉灌注指数（HPI）的差异。结果：（1）20周末共获得S0期、S1期、S2期、S3期、S4期肝纤维化分别为40例、27例、19例、17例、19例。（2）对照组CT灌注参数HAP、PVP、THBF分别为（27.74±11.77,94.70±9.32,122.44±8.79）mL/min·100mL-1;HPI为（20.54±4.08）mL/min·100 mL-1。实验组S0～S4期HAP为（28.24±11.74,18.02±4.70,24.24±9.62,27.45±16.40,32.07±15.77）mL/min·100 mL-1;PVP为（91.69±15.44,77.73±10.52,61.82±10.52,45.08±14.98,36.18±6.22）mL/min·100 mL-1,THBF为（119.93±21.87,95.76±12.09,86.06±11.65,72.54±27.00,68.26±18.91）mL/min·100mL-1;HPI为（22.53±6.25,28.48±5.58,30.82±6.05,37.06±5.89,40.73±10.19）%。各灌注参数对照组与实验组S0期之间比较差异无统计学意义（P〉0.05）;实验组HAP先降后升,S1期与对照组、S3期与S1期、S4期与S2期之间比较差异有统计学意义（P〈0.05）;PVP S0期～S4期呈下降趋势,各组之间比较差异均有统计学意义（均P〈0.05）;THBF S0期～S4期呈下降趋势,S2期与S0期、S3期与S1期、S4期与S2期之间比较差异有统计学意义（P〈0.05）;而HPI升高,各组之间比较差异均有统计学意义（均P〈0.05）。结论：四氯化碳复合因素造模法可以成功建立小型猪肝硬化模型,肝硬化过程中肝脏血流灌注参数的变化与疾病的严重程度有关。

英文摘要：

Objective：To investigate the changes of MSCT blood flow in different pathological stages of minipig liver cirrhosis models preparation.Methods：45syngeneic Bama minipig were randomly divided into the experimental group（n =40）and the control group（n =5）.The minipig liver cirrhosis model in the experimental group was constructed by carbon tetrachloride composite-factor modeling method.Both groups were measured with MSCT perfusion and the liver biopsy after modeling for 4,8,12,16 and 20weeks.Changes of HAP,PVP,THBF and HPI were statistically analyzed in different pathological stages of liver cirrhosis modeling.Results：（1）after 20 weeks,there were 40,27,19,17 and 19cases developed hepatic fibrosis at S0,S1,S2,S3 and S4stage,respectively.（2）HAP,PVP and THBF in control group were（27.74±11.77）,（94.70±9.32）and（122.44±8.79）mL/min·100 mL-1,respectively.HPI were（20.54±4.08）%.From S0 to S4,the HAP in experimental group was（28.24±11.74）,（18.02±4.70）,（24.24±9.62）,（27.45±16.40）and（32.07±15.77）mL/min·100mL-1,respectively.PVP was（91.69±15.44）,（77.73±10.52）,（61.82±10.52）,（45.08±14.98）and（36.18±6.22）mL/min·100 mL-1,respectively,THBF was（119.93±21.87）,（95.76±12.09）,（86.06±11.65）,（72.54±27.00）and（68.26±18.91）mL/min·100mL-1,respectively.HPI was（22.53±6.25）,（28.48±5.58）,（30.82±6.05）,（37.06±5.89）and（40.73±10.19）%,respectively.For CT perfusion parameters,there was no significant difference between the control group and the experimental group in S0（P 〉0.05）.In the experimental group,the HAP descended firstly then ascended,however,the THBF and PVP showed a downward trend,while HPI increased.There were significant differences in HAP,THBF and PVP between the experimental group and the control group（P〈 0.05）.Conclusion：The minipig cirrhosis model could be successfully prepared by carbon tetrachloride composite-factor modeling method,and the changes of MSCT perfusion parameters are corre