中文摘要：

通过对狂犬病病毒鹿源野毒株（DRV）总RNA的提取，用RT—PCR方法扩增磷（NS）蛋白基因并与T载体连接、克隆，将其转化到大肠杆菌JM109细胞中，测定NS蛋白基因的序列，与其他已经发表的国际标准株、疫苗株等进行比较，分析其氨基酸的同源性和NS蛋白的分子生物学功能，为狂犬病病毒野毒株全基因序列的测定奠定基础。

英文摘要：

NS gene was amplified by reverse transcriptase-polymerase chain reaction （RT-PCR） in order to compare NS gene of deer（D） strains. The NS gene sequence among D strains, PV, CVS, SADB19, serotypeI and Mokola V was compared in order to analyse the homology of the six strains as well as study the biological functions of NS protein. All these results laid foundations in sequencing of a complete genome of field rabies virus.