中文摘要：

试验将编码狂犬病病毒糖蛋白、核蛋白融合基因克隆到原核表达载体pET-32a（＋）中，构建狂犬病病毒原核表达质粒。将该质粒转化到大肠杆菌BL21中，经踟诱导表达出约2．09×10^4D的狂犬病病毒糖／核融合基因编码的蛋白质。Western—blotting检测结果表明，该融合蛋白质具有免疫原性。

英文摘要：

The experiment was conducted to construct pmkaryotic expression plasmid containing the G and N melt genes of rabies virus through cloning the genes of rabies virus into pET-32a（ ＋ ）. After transforming the plasmid into E. coli BL21, the bacteria were induced by IPTG and the protein was detected with western-blotting test. The result indicated that the two genes could be co-expressed by the pmkaryotic expression plasmid, which will supply foundation for the preparation of the antigen use to diagnose rabies.