中文摘要：

目的：研究支气管哮喘不同时期细胞外信号调节激酶（External signal regulated kinase,ERK）的磷酸化与c-Fos表达,以探讨ERK信号转导途径在支气管哮喘气道重塑中的作用。方法：复制大鼠哮喘模型,随机分为对照组（包括4周、8周及12周对照组）、哮喘组（包括4周、8周及12周哮喘组）,图像分析软件测定支气管壁厚度（Wat）和平滑肌厚度（Wam）,免疫组化测定肺组织磷酸化的ERK（Phospho-ERK,P-ERK）与c-Fos表达,免疫印迹法测定磷酸化的ERK水平,直线相关分析法显示Wat和Wam与P-ERK的相关性。结果：各哮喘组Wat和Wam,P-ERK和c-Fos的平均吸光度均显著高于相应对照组（P均〈0.01）;各哮喘组磷酸化的ERK水平均显著高于相应对照组（其中A12也与C8组比）（P〈0.01）;Wat、Wam与P-ERK平均吸光度均呈显著正相关性（P〈0.01）。结论：ERK磷酸化水平和c-Fos在哮喘大鼠均增加,ERK信号转导途径在支气管哮喘气道重塑中起重要作用。

英文摘要：

Objective： To study phosphorylafion of ERK and expressions of c-Fos, and to explore the role of external signal regulated kinase （ERK） signal transduction pathway in asthma airway remodeling . Methods：Asthma model was developed,The rats were randomly divided into control groups（ including 4 weeks ,8weeks and 12 weeks control） and asthma groups （including 4 weeks ,8weeks and 12 weeks asthma groups）. Total brochial wall thickness （Wat） and smooth muscle thickness（Warn） were measured by image anal- ysis system. Phospho-ERK（P-ERK） and c-Fos were detected by immunohistochemistry technique;lung tissue extracts were analyzed for phosphorylation of ERK by Western blot. the correlation between Wat and P-ERK,Wam and P-ERK. Results：Wat and Warn,mean optical density（ by immunohistochemistry） of P-ERK and c-Fos in asthma groups were all significantly higher than those in corresponding control groups （P 〈0. 01, respectively） ;the level of P-ERK in asthma groups were significantly higher than that in corresponding control groups （while A12 group also compared with C8 group） （P 〈 0. 01 respectively） ;Wat and Warn positively correlated with mean optical density of P-ERK. Conclusion：The level of P-ERK and c-Fos increase in asthma rats, ERK signal transduction pathway palys an important role in asthma airway remodeling.