中文摘要：

颗粒细胞是卵巢卵泡重要的结构组分,其形态、功能伴随原始卵泡的生长启动、增殖、分化、闭锁、排卵以及黄体形成等生理过程发生各种变化.颗粒细胞是研究细胞增殖、分化和细胞相互作用、信号转导通路的理想细胞模型.利用注射器吸取有腔卵泡卵泡液,成功分离培养了猪有腔卵泡的颗粒细胞,并进行了FSHR免疫荧光检测以及原代猪卵巢颗粒细胞生长活力检测,结果表明：该细胞对FSHR的免疫荧光呈阳性.流式细胞仪检测发现该细胞的细胞周期为24 h,但在培养4d时细胞的活力最强.该研究结果为进一步研究母猪卵巢卵泡功能奠定了基础.

英文摘要：

Granulose cells aer important structural constituent of mammalian ovary, their morphology and function change following the physiological processes of primordial follicular activation, cell proliferation and differentiation, follicular atresia, ovulation and luteinization. At the same time, granulose cell is the usual model to analyze cell interaction and signal transduction of the follicular. Sterile syringes were used to absorb the fluid from the antral follicle. The cells separated and cultured from the follicular fluid were devoted to the FSHR immune-fluorescence identification, cell cycle detection and vitality test. The results demonstrated that cells separated from the antral follicle fluid were granules cells, were positive in the FSHR anti-body. Furthermore, the cell cycle of granulose cell was 24 h, and the most highly cell vitality recched at the 4^th day.