中文摘要：

目的观察抗IL-5抗体（Anti—IL-5Ab）联合抗II。13抗体（Anti—IL-13Ab）对支气管哮喘（简称哮喘）小鼠气道炎症及气道高反应性的影响，为临床应用奠定基础。方法50只雌性Balb／c小鼠随机分成正常组、哮喘组、Anti—IL-5Ab治疗组、AntiIL-13Ab治疗组和Anti—IL-5Ab联合Anti—IL-13Ab治疗组。以清洁级Balb／c小鼠构建哮喘模型，采用腹腔注射卵蛋白和氢氧化铝混悬液处理动物。采用瑞氏染色对哮喘小鼠进行支气管肺泡灌洗液中的细胞分类及计数，应用小鼠肺功能仪检测气道阻力的变化。结果①Anti—IL-5Ab联合AntiIL13Ab治疗组小鼠症状较哮喘组及其他治疗组明显减轻；②Anti—IL-5Ab联合Anti—IL-13Ab治疗组可有效降低哮喘小鼠支气管肺泡灌洗液中嗜酸粒细胞、中性粒细胞、淋巴细胞等炎症细胞水平，与AntiIL5Ab治疗组及AntiIL13Ab治疗组比较差异有统计学意义（P〈i0．05）；③Anti—IL-5Ab联合Anti—IL-13Ab治疗组可有效降低哮喘小鼠气道高反应性，与Anti—IL-5Ab治疗组及Anti—II。-13Ab治疗组比较差异有统计学意义（P〈0．05）。结论Anti—IL-5Ab联合Anti—IL-13Ab治疗组不但可抑制哮喘小鼠肺部嗜酸粒细胞、中性粒细胞、淋巴细胞等炎症细胞浸润，还可以抑制哮喘迟发反应，降低气道高反应性，改善肺功能，为治疗哮喘提供新的解决办法。

英文摘要：

Objective The effects of airway inflammation and airway hyperresponsiveness（AHR） were observed by using anti-IL-5 antibody combined anti-IL-13 antibody in a rat asthmatic model, which was established a foundation for clinical application. Methods Fifty female Balb/c rats were divided randomly into 5 groups： the normal control group, the asthmatic model group, the anti-IL-5 antibody group,the anti-IL-13 antibody group and anti-IL-5 antibody combined anti-IL-13 antibody group. A rat asthmatic model was established. The eosinophil, neutrophil, lymphocyte count in bronchoalveolar lavage fluid （BALF） were made with Wright＇s staining. The change of the airway resistance were analyzed by means of animal lungs function analysis system. Results （1） Onset of asthmatic symptom in anti-IL-5 antibody combined anti-IL-13 antibody group can be relieved compared with the asthmatic model group and other treatment groups. （2）Levels of eosinophil, neutrophil, lymphocyte in BALF were significantly declined in anti-IL-5 antibody combined anti-IL-13 antibody group. There was statistically significant difference between anti-IL-5 antibody combined anti-IL-13 antibody group and other groups on reduction the number of the inflammatory cell in BALF （ P〈0.05）. （3）The level of AHR can be markedly reduced by anti-IL-5 antibody combined anti-IL-13 antibody, which has statistically significant difference comparedwith other treatment groups （ P 〈0.05）. Conclusions Compared with the asthmatic group, anti IL-5 antibody combined anti-IL-13 antibody not only can decrease eosinophil, neutrophil and lymphocyte infiltration but also alleviate AHR and improve pulmonary function in rat asthmatic model,which may be one of effective way to treat asthmatic disease.