运用同一种酶具有两种及其以上酶活力时其活性中心个数的判定方法,对从纤维素酶中分离出的既具有壳聚糖酶活力又具有纤维素酶活力的双功能酶CCBE的活性中心个数进行了研究,结果表明:CCBE系运用同一活性中心催化水解两种不同底物。
A kinetic method was employed to study the active center of the bifunctional enzyme that hydrolyze both carboxymethyl cellulose and chitosan. The experimental data coincide well with the theoretical values calculated from the case of a single active center.