中文摘要：

目的：评估巢氏-PCR和ELISA在巨细胞病毒（HCMV）感染筛查诊断中的价值。方法：以巢式PCR检测新生儿HCMV-DNA为标准,评估ELISA方法检测HCMV-IgG和IgM的诊断价值。结果：采用巢氏PCR方法,患病新生儿HC-MV-DNA检出率为63.900%。以巢式PCR为标准,ELISA-IgM法检测HCMV的灵敏度为3.030%,特异度为100.000%,阳性预测值为100.000%,阴性预测值为29.670%;ELISA-IgG法检测HCMV的灵敏度为36.620%,特异度为42.110%,阳性预测值为70.270%,阴性预测值为15.090%。结论：巢式PCR是筛检HCMV感染的灵敏方法,目前市场上ELISA试剂盒的灵敏度有待提高。

英文摘要：

Objective：To evaluate the values of Nest-PCR and ELISA in screening and diagnosis of cytomegalovirus（HCMV） infection. Methods：Neonatal HCMV DNA detected by Nested PCR was used as standard,the diagnostic value of ELISA in detection of HCMV-IgG and IgM was assessed. Results：The detection rate of HCMV DNA in the neonates detected by Nested PCR was 63.90%,the result of Nested PCR was used as standard,the sensitivity,specificity,positive predictive value and negative predictive value of ELISA-IgM method in detection of HCMV were 3.03%,100.00%,100.00% and 29.67%,respectively;the sensitivity,specificity,positive predictive value and negative predictive value of ELISA-IgG method in detection of HCMV were 36.62%,42.11%,70.27% and 15.09%,respectively. Conclusion：Nest-PCR is a method to screen HCMV infection with high sensitivity,at present,the sensitivity of ELISA kit on the market need to be increased.