中文摘要：

The present experiment was performed with the objective of examining the effects of copper sources and levels on hydrogen peroxide（H₂O₂） generation by mitochondria from broiler hepatocytes. Treatments were applied to compare sources of copper（CuSO₄ versus Cu-Met） and 4 levels of dietary Cu （11,110,220 and 330 mg/kg）.Day-old broilers（Cobb 500,Gallus domesticus,n=288） were randomly divided into 8 groups of 36 each and fed diets as follows:Controls（Cu 11 mg/kg） and high copper（Cu 110, 220,and 330 mg/kg）,for 60 days under normal conditions.Sample collections were made at 12,36 and 60 days of age to investigate the changes in H₂O₂ generation by mitochondria from hepatocytes.Compared with those of the control diets,H₂O₂ generation by mitochondria in the high copper groups（110 to 330 mg/kg） of the two copper sources were increased（P【0.05 or P【0.01）;At days 36 and 60,H₂O₂ generation by hepatic mitochondria from Cu-Met supplementation exceeded that from birds supplemented with CuSO₄ （P【0.05 or P【0.01）.In addition,H₂O₂ generation by mitochondria from broilers fed with high dietary copper appeared to be associated with altered function of mitochondrial complexⅣ.The results indicated that dietary supplementation with copper induced oxidative stress damage in liver.At each level of copper supplementation,the organic Cu-Met led to more rapid H₂O₂ generation than did inorganic CuSO₄.The results also suggest that mitochondrial complexⅣmay be targeted under conditions of high dietary copper supplementation.