中文摘要：

在本文中,进一步发展了以二联吡啶二吡啶并[3,2-a：2＇,3＇-c]吩嗪钌（[Ru（bpy）2dppz]^2＋为电化学发光指示剂的DNA损伤传感器。将通过S-Au键组装在金电极表面的双链DNA浸泡在[Ru（bpy）2dppz]^2＋溶液中,利用[Ru（bpy）2dppz]^2＋是双链DNA分子＂光开关＂的特性,得到[Ru（bpy）2dppz]^2＋/DNA/Au电化学发光传感器,并优化了[Ru（bpy）2dppz]^2＋的浸泡时间,浸泡浓度等实验条件。发现在含有[Ru（bpy）2dppz]^2＋的底液中测定其电化学发光强度值比不含[Ru（bpy）2dppz]^2＋的底液中增强了6倍左右。此外将此传感器经全氟辛酸磺酸、五溴联苯醚、氧化苯乙烯等物质温浴后,其电化学发光强度显著降低,说明该传感器可用于环境污染物对DNA损伤的筛查测定。

英文摘要：

In this article,a DNA damage sensor was further developed by using[Ru（ bpy）2dppz]^2＋as electrochemiluminescence（ ECL） indicator.The thiol-labeled ds DNA was assembled on a Au electrode via Au-S bond and then the electrode was immersed in[Ru（ bpy）2dppz]^2＋solutions.A[Ru（ bpy）2dppz]^2＋/DNA/Au ECL sensor was obtained based on[Ru（ bpy）2dppz]^2＋as ds DNA molecular＂light switch＂. Moreover,the experimental conditions,such as[Ru（ bpy）2dppz]^2＋soaking time,soaking concentrations were optimized.The ECL intensity measured in substrate solution containing[Ru（ bpy）2dppz]^2＋increased five times than the substrate solution absence of[Ru（ bpy）2dppz]^2＋. What＇s more,the ECL intensity was significantly reduced when the sensor was incubated in pentabromodiphenyl ether,styrene oxide,2-aminobenzimidazole,quinclorac solutions,indicating that the prepared sensor can be used for environmental pollutants screening measurement of DNA damage.